Observing Bacteria Essay Example for Free

detect Bacteria EssayAbstract Microscopes are fragile instruments that must be handle with extreme management as they can produce high bore results when observant the smallest exemplars on earth. A microscope must be properly cleaned before use and storage. The different objectives go out for a range of observations. At the highest objectives, the resolution can easily be lost which is why the oil immersion lens is used to minimize refraction. speckle observing specimens, both dead and active, their shapes and arrangements can be observed. For example, the oil immersion lens can be used to observe the active Streptobacillus bacteria in yogurt.Purpose The purpose of this lab was to display knowledge of the use of a compound microscope with and without an oil immersion lens term observing and identifying various bacterial shapes and arrangements, including a self prepared yogurt culture.ProcedureExercise 1 Viewing Prepared Slides1. After setting up and cleaning all lenses, rear the prepared e slide properly on the confront. 2. View the slide under the10x objective by moving it around with the X and Y stage travel knobs then tension it by first using the coarse adjustment followed by the fine adjustment until the view is clear. 3. Adjust the diaphragm to lease enough light for good resolution. 4. After a micrograph is taken, rotate the 10x objective away from the specimen and the 40x over it. Use the fine adjustment knob to submit the specimen back into focus. 5. Repeat the above stairs for 6 more specimens. Those viewed and micrographed in this lab are amoebaAnabaenaAscaris eggsParamecium barmPenicillium lift off 2 of Exercise 11. View 6 more prepared slides by using the oil immersion lens. Follow steps 1-5 above to locate, center, and focus each slide at 10x and 40x. 2. Then swing the 40x objective to its half way position with the 100x objective nearing the slide. 3. Add a swing over of provided oil to the slide covers surface and slowly swing the 100x objective over. 4. Using the fine adjustment knob bring the specimen into focus and take a micrograph. 5. Repeat this process for 5 more specimens. Those that were micrograph in this lab wereBacillusCoccusSpirilliumPrepared YogurtExercise 2 Observing Bacteria Cultures in Yogurt1. Using a clean sealable glass jar, seat a teaspoon of yogurt in the container. 2. Cover the jar and place in a dark, relatively warm areas fro 12-24 hours. 3. Place a sample of the yogurt specimen in a clean slide using a toothpick and cover with a cover slip. 4. Repeat the above steps for viewing the slide at the 10x, 40x, and 100x oil immersion with the microscope. Keep the diaphragm low, as the bacteria will be transparent. 5. Repeat the viewing process with the prepared yogurt slide from the lab kit. Compare the two specimens. 6. Clean all items used in this lab specimen vials, slides, and microscope. Carefully cover and store microscope.Data/ObservationSpecimens observed in Exercise 1 Part 1 with the 10x objectiveAmoeba Proteus at 100xAnabaena w.m. at 100xAscaris Eggs, w.m. at 100xParamecium Conjugation at 100xYeast, w.m. at 100xPencillium with conidia, w.m. at 100xSpecimens observed in Exercise 1 Part 2 Exercise 2 with the 40x objectiveBacteria Bacillus variety show at 400xBacteria Coccus form at 400xBacteria spirillum form at 400x Yoghurt Bacteria at 400x accented Yogurt Specimen at 400xSpecimens observed in Exercise 2 with the 100x Oil ImmersionFresh Yogurt Specimen at 1000xResults/AnalysisA. Identify the following parts of the microscope and describe the function of each.A. Eyepiece lensB. TubeC. NosepieceD. Objective electron lensE. StageF. DiaphragmG. MirrorH. Coarse Adjustment knobI. Fine Adjustment knobJ. ArmK. ClipL. BaseB. Define the following microscopy footingFocus The point at which the light from a lens comes together.Resolution The closest two objects can be before they are no thirster detected as separate objects.Contrast The difference in light in tensity between the stunt man and the adjacent accentuate relative to the overall background intensity.C. Describe your observations from the fresh yogurt slide you prepared in Exercise 2. D. Were there observable differences between your fresh yogurt slide and the prepared yogurt slide? If so, explain.CD Observation of the prepared slide was made easier by the purple staining and the dead bacteria. The observation of the fresh specimen was harder to see in a focused manner because it continued to move. The prepared slide is an obvious Bacillus bacterium. While the fresh sample is harder to focus so it is not as easily observed as Bacillus bacterium. There are more bacteria present in the fresh specimen than in the prepared slide.E Describe the four main bacterial shapes.Cocci a spherically shaped bacteriumBacillus a rod shaped bacteriumSpirillum spirally shaped bacteriumVibrio comma or S shaped bacteriaF. What are the common arrangements of bacteria?Cocci occurring as a undivide d sphereDiplococci occurring as spheres in pairsStreptococci chains of linked spheresStaphylococci spheres grouped in grape like clustersBacillus a single rod arrangementDiplobacillus pairs of rodsStreptobacillus Chain-linked rodsG. Were you able to identify specific bacterial morphologies on either yogurt slide? If so, which types?In both samples Streptobacilluss arrangements and shapes were observed. They were more prevalent and escaped to distinguish in the prepared slide since that bacteria was not actively moving. At times the fresh specimen at 400x looked like Staphylococci however, upon observing the bacteria closer at 1000x it was obvious that there were no sphere shaped bacterium present.H. What is the purpose of immersion oil? Why does it work?Normally, the quality of an observed specimen decreases with the number of lenses, glass, etc that the light travels through. With the oil immersion lens, one of the strongest microscope lenses at 100x, the oil restricts the light r efraction allowing for a clear focused image at such a high resolution. The oil mixes with the specimen and the oil itself has the same refractive index to that of glass. This gives the specimen a finer resolution and stylishness than would have otherwise been observed.Conclusion In conclusion this lab taught one how to properly use, clean, and store compound microscope. The lab assessed the ability to observe a specimen both provided and freshly prepared with and without an oil immersion lens. This allowed for hands on learning, observation, and identification of various bacterial shapes and arrangements. One was able to extended his/her learning while ageing yogurt and making a fresh specimen on a slide to be observed at all objectives with and without oil.